Qubit dsDNA HS Assay Kit /Qubit dsDNA HS定量试剂盒
Qubit dsDNA HS Assay Kit双链DNA高灵敏度荧光定量试剂盒限与Qubit荧光计一起使用。该测定对双链DNA(dsDNA)是高度选择性的,并设计为对初始样品浓度从10 pg /μL到100 ng /μL是准确的。该试剂盒提供浓缩的定量试剂,稀释缓冲液和预稀释的DNA标准品。只需使用提供的缓冲液稀释试剂,添加样品(1μl—20μl),并使用Qubit荧光计读取浓度。定量试剂对常见的污染物,如盐,游离核苷酸,溶剂,去污剂或蛋白质都有良好的耐受性。
HS (High Sensitivity):高灵敏度
搭配仪器
对于1-20个样品:Qubit dsDNA HS定量试剂盒和Qubit荧光仪一起使用
对于20-2000个样品:Quant-iT dsDNA HS定量试剂盒和酶标仪(microplate reader)一起使用
注意:
1.所有Qubit定量试剂盒都可以与Qubit 1.0,Qubit 2.0,Qubit 3和Qubit 4荧光计一起使用。
2.需要500μL薄壁PCR管(Q32856),但不包括在内。
3.也可作为1X dsDNA定量试剂盒,配好的试剂可作为即用型溶液稳定长达一年。
产品特点:
核心范围(高可信度):1 ng / mL至500 ng / mL
扩展范围(中等可信度):0.5 ng / mL至1 ng / mL和500 ng / mL至600 ng / mL
用于定量PCR产物、病毒DNA和亚克隆(subcloning)样品
在RNA,盐,溶剂,蛋白质和游离核苷酸的存在下是准确的
规格
样本类型 | dsDNA |
使用仪器 | Qubit® Fluorometer荧光定量仪 |
定量范围 | 0.2-100 ng |
反应次数 | 100 次 / 500 次 |
绿色环保 | Fewer resources used, Less waste |
运输条件 | 室温 |
订购信息
品牌 | 产品描述 | 货号 | 规格 |
Invitrogen | Qubit™ dsDNA HS Assay Kit | Q32851 | 100 assays |
Invitrogen | Qubit™ dsDNA HS Assay Kit | Q32854 | 500 assays |
The Qubit dsDNA HS (High Sensitivity) Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.
Which product to choose for dsDNA HS (High Sensitivity) quantitation?
• For 1–20 samples: use this Qubit dsDNA HS Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT dsDNA HS Assay Kit with microplate reader
适用于(设备): | Qubit® Fluorometer |
---|---|
样品类型: | dsDNA |
页反应的: | 500 reactions |
数量: | 500 assays |
Quantitation Range: | 0.2-100 ng |
Shipping Condition: | Room Temperature |
Product Line: | Quant-iT™, Qubit™ |
Q32854 | 500 assays |
Store reagent and buffer at room temperature (protect reagent from light). Store the standards at 2–8°C.