{"id":1077,"date":"2021-04-22T09:42:59","date_gmt":"2021-04-22T01:42:59","guid":{"rendered":"http:\/\/www.dsunbio.com\/?p=1077"},"modified":"2021-04-22T09:42:59","modified_gmt":"2021-04-22T01:42:59","slug":"taqman-fast-advanced%e9%a2%84%e6%b7%b7%e6%b6%b2","status":"publish","type":"post","link":"http:\/\/www.dsunbio.com\/?p=1077","title":{"rendered":"TaqMan\u2122 Fast Advanced\u9884\u6df7\u6db2"},"content":{"rendered":"

TaqMan\u2122 Fast Advanced\u9884\u6df7\u6db2<\/strong><\/p>\n

\"\"<\/p>\n

TaqMan Fast Advanced \u9884\u6df7\u6db2\u5728\u4e00\u7cfb\u5217 qPCR \u4eea\u5668\u5e73\u53f0\u4e0a\u4ee5\u66f4\u77ed\u7684\u65f6\u95f4\u63d0\u4f9b\u51c6\u786e\u7684\u5b9a\u91cf\u548c\u53ef\u9760\u6027\u3002\u5373\u4f7f\u662f\u5177\u6709\u6311\u6218\u6027\u7684\u9776\u6807\uff0c\u5b83\u4e5f\u80fd\u5728\u5355\u6b21\u53cd\u5e94\u548c\u53cc\u91cd\u53cd\u5e94\u4e2d\u63d0\u4f9b\u4e00\u6d41\u7684\u6027\u80fd\uff0c\u5305\u62ec\u8f83\u5927\u7684\u53ef\u91cf\u5316\u8303\u56f4\u3002TaqMan Fast Advanced \u9884\u6df7\u6db2\u5305\u542b AmpliTaq Fast DNA \u805a\u5408\u9176\u3001\u5c3f\u5627\u5576-N-\u7cd6\u57fa\u5316\u9176 (UNG)\u3001\u52a0\u5165 dUTP \u7684 dNTP\u3001ROX \u67d3\u6599\uff08\u88ab\u52a8\u53c2\u6bd4\uff09\u548c\u4f18\u5316\u7684\u7f13\u51b2\u6db2\u7ec4\u5206\u3002\u5b83\u4ee52X \u6d53\u5ea6\u63d0\u4f9b\u3002<\/p>\n

TaqMan Fast Advanced \u9884\u6df7\u6db2\u7684\u7279\u70b9\u5305\u62ec\uff1a
\n\u2022\u51fa\u8272\u7684\u6027\u80fd<\/b>\u2014\u2014\u4e0e\u6807\u51c6\u6a21\u5f0f\u4e0b\u7684\u6807\u51c6\u6df7\u5408\u6db2\u76f8\u6bd4\uff0c\u5177\u6709\u66f4\u4f18\u7684\u7075\u654f\u5ea6\u3001\u51c6\u786e\u5ea6\u3001\u52a8\u6001\u8303\u56f4\u548c\u7279\u5f02\u6027
\n\u2022\u00a0\u4e13\u4e3a\u589e\u5f3a\u53f0\u5f0f\u7a33\u5b9a\u6027\u800c\u8bbe\u8ba1<\/b>\u2014\u2014\u5728\u9884\u6784\u5efa\u53cd\u5e94\u4e2d\uff0c\u5ba4\u6e29\u4e0b\u53ef\u7a33\u5b9a\u957f\u8fbe72\u5c0f\u65f6
\n\u2022\u00a0\u9488\u5bf9\u591a\u91cd\u53cd\u5e94\u8fdb\u884c\u4e86\u4f18\u5316<\/b>\u9488\u5bf9\u5916\u6e90\u6027\u548c\u5185\u6e90\u6027\u5185\u90e8\u9633\u6027\u5bf9\u7167Assay\u7684\u53cc\u91cd\u6027\u8fdb\u884c\u4e86\u9a8c\u8bc1
\n\u2022\u00a0\u7f29\u77ed\u5feb\u901f\u548c\u6807\u51c6\u4eea\u5668\u7684\u8fd0\u884c\u65f6\u95f4\uff08<40\u5206\u949f\uff09<\/b>\u2014\u2014\u9488\u5bf9\u5feb\u901f\u4eea\u5668\u7684\u5feb\u901f\u6a21\u5f0f\u548c\u6807\u51c6\u4eea\u5668\u7684\u5feb\u901f\u5faa\u73af\u6761\u4ef6\u8fdb\u884c\u4e86\u4f18\u5316
\n\u2022\u00a0\u65e0\u7f1d\u5730\u8fc7\u6e21\u5230\u5b9e\u9a8c<\/b>\u2014\u2014\u901a\u8fc7 TaqMan \u57fa\u56e0\u8868\u8fbe\u548c microRNA Assay\u4ee5\u53ca TaqMan \u9635\u5217\u5fae\u6d41\u4f53\u5361\u8fdb\u884c\u4e86\u9a8c\u8bc1<\/p>\n

\u51fa\u8272\u7684\u6027\u80fd<\/b>
\nTaqMan Fast Advanced \u9884\u6df7\u6db2\u65e8\u5728\u63d0\u4f9b\u4e0e\u6807\u51c6\u9884\u6df7\u6db2\u76f8\u5f53\u6216\u66f4\u597d\u7684\u6027\u80fd\u3002\u76ee\u524d\u5df2\u9488\u5bf9\u9886\u5148\u4f9b\u5e94\u5546\u7684\u6807\u51c6\u548c\u5feb\u901f\u9884\u6df7\u6db2\u5bf9\u5176\u8fdb\u884c\u4e86\u57fa\u51c6\u6d4b\u8bd5\uff0c\u4ee5\u5e2e\u52a9\u786e\u4fdd\u59a5\u5584\u63d0\u4f9b\u4e00\u6d41\u7684\u7075\u654f\u5ea6\u3001\u51c6\u786e\u5ea6\u3001\u52a8\u6001\u8303\u56f4\u548c\u7279\u5f02\u6027\u3002TaqMan Fast Advanced \u9884\u6df7\u6db2\u7684\u52a8\u6001\u8303\u56f4\uff08\u591a\u8fbe7\u4e2a\u65e5\u5fd7\uff09\u662f\u4e1a\u754c\u8f83\u5e7f\u6cdb\u7684\u3002\u4e0b\u56fe\u5728\u4e00\u7ec4\u57fa\u56e0\u8868\u8fbeAssay\u4e2d\u6bd4\u8f83\u4e86 TaqMan Fast Advanced \u9884\u6df7\u6db2\u548c TaqMan Universal PCR \u9884\u6df7\u6db2\u7684 Ct \u503c\uff0c\u5c55\u793a\u4e86\u8be5\u9884\u6df7\u6db2\u51fa\u8272\u7684\u7075\u654f\u5ea6\u3002<\/p>\n

\u53f0\u5f0f\u7a33\u5b9a\u6027\uff0c\u5b9e\u73b0\u9ad8\u901a\u91cf\u5904\u7406\u548c\u4fbf\u6377\u6027<\/b>
\nTaqMan Fast Advanced \u9884\u6df7\u6db2\u7ecf\u8fc7\u7cbe\u5fc3\u8bbe\u8ba1\uff0c\u53ef\u5728\u9884\u6784\u5efa\u53cd\u5e94\u4e2d\u4fdd\u6301\u957f\u8fbe72\u5c0f\u65f6\u7684\u9ad8\u6c34\u5e73\u6027\u80fd\u3002\u6b64\u9884\u6df7\u6db2\u5177\u6709\u9ad8\u7a33\u5b9a\u6027\uff0c\u53ef\u5411\u9ad8\u901a\u91cf\u6db2\u4f53\u5904\u7406\u7cfb\u7edf\u7684\u7528\u6237\u4fdd\u8bc1\u7b2c\u4e00\u68c0\u6d4b\u677f\u7684\u7ed3\u679c\u4e0e\u6700\u540e\u4e00\u4e2a\u68c0\u6d4b\u677f\u7684\u7ed3\u679c\u76f8\u4eff\u3002\u4e0b\u56fe\u663e\u793a\u4e86\u5728\u6784\u5efa\u65f6\uff08\u65f6\u95f40\uff09\u548c\u572830\u00b0C \u4e0b\u57f9\u517b72\u5c0f\u65f6\u540e\u8fdb\u884c\u7684Assay\uff0c\u6a21\u62df\u4e86\u8f83\u6781\u7aef\u5ba4\u6e29\u573a\u666f\u3002<\/p>\n

\u5bf9\u4e8e\u6ca1\u6709\u6781\u9ad8\u901a\u91cf\u9700\u6c42\u7684\u7528\u6237\uff0c\u6b64\u9884\u6df7\u6db2\u589e\u5f3a\u4e86\u7a33\u5b9a\u6027\uff0c\u4e3a\u60a8\u7684\u5de5\u4f5c\u6d41\u7a0b\u989d\u5916\u63d0\u4f9b\u4e86\u6574\u4f53\u4e0a\u7684\u4fbf\u5229\uff0c\u56e0\u4e3a\u60a8\u4e0d\u518d\u53d7\u9650\u4e8e\u6784\u5efa\u540e\u9700\u7acb\u5373\u8fd0\u884c\u68c0\u6d4b\u677f\u3002<\/p>\n

\u9488\u5bf9\u591a\u91cd\u53cd\u5e94\u8fdb\u884c\u4e86\u4f18\u5316<\/b>
\n\u6211\u4eec\u610f\u8bc6\u5230\uff0c\u4fe1\u5fc3\u5bf9\u60a8\u7684\u7ed3\u679c\u81f3\u5173\u91cd\u8981\u3002\u4e3a\u4e86\u589e\u52a0\u6bcf\u4e2a\u5b54\u4e2d\u6240\u89c1\u7ed3\u679c\u7684\u4fe1\u5fc3\uff0cTaqMan Fast Advanced \u9884\u6df7\u6db2\u8bbe\u8ba1\u4f7f\u7528\u5185\u90e8\u9633\u6027\u5bf9\u7167 (IPC) \u4e3a\u53cc\u91cd\u53cd\u5e94\u63d0\u4f9b\u51c6\u786e\u7684\u7ed3\u679c\u3002\u4e0b\u56fe\u663e\u793a\u4e86\u5b9e\u9a8c\u9776\u6807 \u03b2-\u808c\u52a8\u86cb\u767d\uff08\u57fa\u56e0\u540d\u79f0\uff1aACTB\uff09\u7684\u7ed3\u679c\uff0c\u5176\u5728\u5355\u9776\u6807\u53cd\u5e94\u548c\u53cc\u91cd\u53cd\u5e94\u4e2d\u8fde\u7eed\u7a00\u91ca\u548c\u6269\u589e\u3002<\/p>\n

\u5df2\u901a\u8fc7 microRNA Assay\u9a8c\u8bc1<\/b>
\nTaqMan Fast Advanced \u9884\u6df7\u6db2\u5df2\u7ecf\u8fc7\u9a8c\u8bc1\uff0c\u53ef\u7528\u4e8e\u591a\u79cd\u5b9e\u65f6 PCR \u5e94\u7528\uff0c\u5305\u62ec microRNA Assay\u3002\u8be5\u914d\u65b9\u63d0\u4f9b\u4e86\u8f83\u9ad8\u7684\u7279\u5f02\u6027\u548c\u52a8\u6001\u8303\u56f4\uff0c\u8fd9\u4e24\u4e2a\u8f83\u5173\u952e\u7684\u6027\u80fd\u5b9a\u4e49\u4e86\u5904\u7406 microRNA \u65f6\u7684\u6210\u529f\u7ed3\u679c\u3002\u4e0b\u56fe\u4e2d\u7684\u6570\u636e\u8868\u660e\uff0c\u5728\u8f93\u5165\u6a21\u677f\u76846-\u5bf9\u6570\u8303\u56f4\u5185\uff0cPCR \u5177\u6709\u51fa\u8272\u7684\u7ebf\u6027\u3002<\/p>\n

\u7f29\u77ed\u6807\u51c6\u4eea\u5668\u7684\u8fd0\u884c\u65f6\u95f4<\/b>
\nTaqMan Fast Advanced \u9884\u6df7\u6db2\u5df2\u7ecf\u8fc7\u4f18\u5316\uff0c\u53ef\u7528\u4e8e\u5feb\u901f\u548c\u6807\u51c6\u4eea\u5668\uff0c\u4f7f\u76ee\u524d\u62e5\u6709\u6807\u51c6\u4eea\u5668\u7684\u7814\u7a76\u4eba\u5458\u4e5f\u80fd\u83b7\u5f97\u8fd9\u79cd\u9884\u6df7\u6db2\u63d0\u4f9b\u7684\u6027\u80fd\u4f18\u52bf\u548c\u8282\u7701\u7684\u65f6\u95f4\u3002<\/p>\n

\n

\u89c4\u683c<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u9002\u7528\u4e8e\uff08\u8bbe\u5907\uff09:<\/th>\n7500Fast System,7500 System,7900HT System, QuantStudio\u2122 12k Flex,QuantStudio\u2122 3,QuantStudio\u2122 5,QuantStudio\u2122 6 Flex,QuantStudio\u2122 7,StepOne\u2122,StepOnePlus\u2122,ViiA\u2122 7 System<\/td>\n<\/tr>\n
\u5f15\u7269\u63a2\u9488\u517c\u5bb9\u6027:<\/th>\nTaqMan\u2122<\/td>\n<\/tr>\n
\u4ea7\u54c1\u7ebf:<\/th>\nTaqMan\u2122<\/td>\n<\/tr>\n
\u6570\u91cf:<\/th>\n1 x 5 mL<\/td>\n<\/tr>\n
\u6837\u54c1\u7c7b\u578b:<\/th>\nDNA\uff08\u57fa\u56e0\u7ec4\uff09\u3001cDNA<\/td>\n<\/tr>\n
\u8fd0\u8f93\u6761\u4ef6:<\/th>\n\u5e72\u51b0<\/td>\n<\/tr>\n
\u6d53\u7f29:<\/th>\n2 X<\/td>\n<\/tr>\n
\u68c0\u6d4b\u65b9\u6cd5:<\/th>\n\u5f15\u7269-\u63a2\u9488\u68c0\u6d4b<\/td>\n<\/tr>\n
Fidelity (vs. Taq):<\/th>\n2 X<\/td>\n<\/tr>\n
\u9002\u7528\u4e8e\uff08\u5e94\u7528\uff09:<\/th>\nReal Time PCR (qPCR)<\/td>\n<\/tr>\n
\u5f62\u5f0f:<\/th>\nLiquid<\/td>\n<\/tr>\n
\u4ea7\u54c1\u89c4\u683c:<\/th>\nLiquid<\/td>\n<\/tr>\n
GC-Rich PCR Performance:<\/th>\nHigh<\/td>\n<\/tr>\n
No. of Reactions:<\/th>\n500\u6b21\u53cd\u5e94<\/td>\n<\/tr>\n
PCR \u65b9\u6cd5:<\/th>\nqPCR<\/td>\n<\/tr>\n
\u805a\u5408\u9176:<\/th>\nAmpliTaq\u2122 \u5feb\u901f DNA \u805a\u5408\u9176<\/td>\n<\/tr>\n
Product Type Specs:<\/th>\nFast Advanced Master Mix<\/td>\n<\/tr>\n
\u53cd\u5e94\u901f\u5ea6:<\/th>\n\u5feb\u901f<\/td>\n<\/tr>\n
\u5bb9\u91cf:<\/th>\n5 mL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\n

\u5185\u5bb9\u53ca\u50a8\u5b58<\/h2>\n
\u5305\u542b1\u7ba1 (5 ml) 2X TaqMan\u2122 Fast Advanced \u9884\u6df7\u6db2\uff08\u8db3\u4ee5\u8fdb\u884c500\u6b21\u53cd\u5e94\uff09<\/p>\n

TaqMan\u2122 Fast Advanced \u9884\u6df7\u6db2\u5305\u542b AmpliTaq\u2122 Fast DNA \u805a\u5408\u9176\u3001\u5c3f\u5627\u5576-N \u7cd6\u57fa\u5316\u9176 (UNG)\u3001dNTP\uff08\u542b dUTP\uff09\u3001ROX\u2122 \u67d3\u6599\uff08\u88ab\u52a8\u53c2\u6bd4\uff09\u548c\u4f18\u5316\u7f13\u51b2\u6db2\u3002<\/p>\n

\u6536\u5230\u540e\uff0c\u4e8e -20\u00b0C \u50a8\u5b58\u3002\u6253\u5f00\u540e\uff0c\u4e8e4\u00b0C \u50a8\u5b58\u3002<\/p><\/div>\n<\/div>\n

<\/div>\n
\u00a0 \u8d27\u53f7\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u5355\u4f4d\u89c4\u683c
\n4444557\u00a0 \u00a0 \u00a01 x 5 mL
\n4444556\u00a0 \u00a0 \u00a01 x 1 mL
\n4444963\u00a0 \u00a0 \u00a02 x 5 mL
\n4444964\u00a0 \u00a0 \u00a05 x 5 mL
\n4444965\u00a0 \u00a0 \u00a010 x 5 mL
\n4444558\u00a0 \u00a0 \u00a01 x 50 mL<\/div>\n","protected":false},"excerpt":{"rendered":"

TaqMan\u2122 Fast Advanced\u9884\u6df7\u6db2<\/strong><\/p>\n

TaqMan Fast Advanced \u9884\u6df7\u6db2\u5728\u4e00\u7cfb\u5217 qPCR \u4eea\u5668\u5e73\u53f0\u4e0a\u4ee5\u66f4\u77ed\u7684\u65f6\u95f4\u63d0\u4f9b\u51c6\u786e\u7684\u5b9a\u91cf\u548c\u53ef\u9760\u6027\u3002\u5373\u4f7f\u662f\u5177\u6709\u6311\u6218\u6027\u7684\u9776\u6807\uff0c\u5b83\u4e5f\u80fd\u5728\u5355\u6b21\u53cd\u5e94\u548c\u53cc\u91cd\u53cd\u5e94\u4e2d\u63d0\u4f9b\u4e00\u6d41\u7684\u6027\u80fd\uff0c\u5305\u62ec\u8f83\u5927\u7684\u53ef\u91cf\u5316\u8303\u56f4\u3002TaqMan Fast Advanc [Read More\u2026]<\/a><\/span><\/p>\n

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